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Marion Cardinaud

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titre
The early stages of the immune response of the European abalone Haliotis tuberculata to a Vibrio harveyi infection.
auteur
Marion Cardinaud, Nolwenn M. Dheilly, Sylvain Huchette, Dario Moraga, Christine Paillard
article
Developmental and Comparative Immunology, Elsevier, 2015, 51 (2), pp.287-297. ⟨10.1016/j.dci.2015.02.019⟩
annee_publi
2015
resume
Vibrio harveyi is a marine bacterial pathogen responsible for episodic abalone mortalities in France, Japan and Australia. In the European abalone, V. harveyi invades the circulatory system in a few hours after exposure and is lethal after 2 days of infection. In this study, we investigated the responses of European abalone immune cells over the first 24 h of infection. Results revealed an initial induction of immune gene expression including Rel/NF-kB, Mpeg and Clathrin. It is rapidly followed by a significant immunosuppression characterized by reduced cellular hemocyte parameters, immune response gene expressions and enzymatic activities. Interestingly, Ferritin was overexpressed after 24 h of infection suggesting that abalone attempt to counter V. harveyi infection using soluble effectors. Immune function alteration was positively correlated with V. harveyi concentration. This study provides the evidence that V. harveyi has a hemolytic activity and an immuno-suppressive effect in the European abalone.
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titre
Vibrio harveyi Adheres to and Penetrates Tissues of the European Abalone Haliotis tuberculata within the First Hours of Contact
auteur
Marion Cardinaud, Annaïck Barbou, Carole Capitaine, Adeline Bidault, Antoine Marie Dujon, Dario Moraga, Christine Paillard
article
Applied and Environmental Microbiology, American Society for Microbiology, 2014, 80 (20), pp.6328-6333. ⟨10.1128/AEM.01036-14⟩
annee_publi
2014
resume
Vibrio harveyi is a marine bacterial pathogen responsible for episodic epidemics generally associated with massive mortalities in many marine organisms, including the European abalone Haliotis tuberculata. The aim of this study was to identify the portal of entry and the dynamics of infection of V. harveyi in the European abalone. The results indicate that the duration of contact be-tween V. harveyi and the European abalone influences the mortality rate and precocity. Immediately after contact, the epithelial and mucosal area situated between the gills and the hypobranchial gland was colonized by V. harveyi. Real-time PCR analyses and culture quantification of a green fluorescent protein-tagged strain of V. harveyi in abalone tissues revealed a high density of bacteria adhering to and then penetrating the whole gill-hypobranchial gland tissue after 1 h of contact. V. harveyi was also de-tected in the hemolymph of a significant number of European abalones after 3 h of contact. In conclusion, this article shows that a TaqMan real-time PCR assay is a powerful and useful technique for the detection of a marine pathogen such as V. harveyi in mollusk tissue and for the study of its infection dynamics. Thus, we have revealed that the adhesion and then the penetration of V. harveyi in European abalone organs begin in the first hours of contact. We also hypothesize that the portal of entry of V. har-veyi in the European abalone is the area situated between the gills and the hypobranchial gland.
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https://hal.univ-brest.fr/hal-01104233/file/Appl.%20Environ.%20Microbiol.-2014-Cardinaud-6328-33.pdf BibTex
titre
The impacts of handling and air exposure on immune parameters, gene expression, and susceptibility to vibriosis of European abalone Haliotis tuberculata.
auteur
Marion Cardinaud, Clément Offret, Sylvain Huchette, Dario Moraga, Christine Paillard
article
Fish and Shellfish Immunology, Elsevier, 2014, 36 (1), pp.1-8. ⟨10.1016/j.fsi.2013.09.034⟩
annee_publi
2014
resume
Wild or farmed abalone are regularly exposed to stressors, such as air exposure and handling. Immune and transcriptional responses as well as susceptibility to vibriosis of sexually mature or immature European abalone acclimated at 16 or 19 °C were determined following handling or air exposure. Hemocyte density and H2O2 production increased while hemocyte viability and phagocytic index decreased following handling. Air exposure induces a decrease of hemocyte density and phagocytic index. Measurement of the expression of genes implicated in general metabolic, immunological and stress responses in gills, foot-muscle and hemocytes by real time q-PCR suggested that both stressors lead to a metabolic rate depression, characterized by a general inhibition of transcription. Finally, following handling a Vibrio harveyi challenge enhances almost 100% mortality of sexually immature animals at 19 °C while it has been previously demonstrated that only mature are susceptible to vibriosis.
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titre
Étude multifactorielle de la vibriose chez l'ormeau européen Haliotis tuberculata : bases génomiques et physiologiques de la survie aux mortalités estivales chez l'ormeau européen Haliotis tuberculata
auteur
Marion Cardinaud
article
Zoologie des invertébrés. Université de Bretagne occidentale - Brest, 2013. Français. ⟨NNT : 2013BRES0062⟩
annee_publi
2013
resume
Depuis une quinzaine d’années, des mortalités estivales d’ormeaux européens, Haliotis tuberculata, surviennent sur le littoral breton et normand, et en structures aquacoles. Ces mortalités sont attribuées à l’espèce bactérienne Vibrio harveyi, et se produisent chez des ormeaux sexuellement matures lorsque la température de l’eau dépasse 17°C.Ce travail de thèse visait en une approche multifactorielle de l’étude de cette interaction hôte-parasite, afin de spécifier les conditions intrinsèques aux ormeaux dans le déclenchement de cette vibriose, le cycle infectieux de V. harveyi chez l’ormeau européen et le rôle de la température dans l’accomplissement de ce cycle infectieux, et enfin la réponse physiologique de l’ormeau lors d’une exposition à V. harveyi.Les principaux résultats montrent un différentiel d’expression génomique entre des ormeaux résistants et des ormeaux sensibles au cours d’une exposition à V. harveyi, attestant ainsi l’importance du statut physiologique de l’hôte dans la survie à la vibriose chez l’ormeau européen. Ce constat est supplémenté de la mise en évidence de sensibilité à cette maladie chez des ormeaux sexuellement immatures, habituellement résistants, acclimatés à 19°C et exposés à des conditions contraignantes de type manipulation. Par ailleurs, l’étude de la voie d’entrée et de la dynamique d’infection de V. harveyi chez l’ormeau européen a révélé un tropisme particulier de ce vibrion pathogène vers les tissus branchiaux dès les premières heures de contact, et son invasion dans le système circulatoire dès 24h de contact. L’étude de la réponse hémocytaire des ormeaux et du métabolisme branchial, à l’échelle moléculaire et cellulaire, lors des premières heures de contact, démontre 1/ la genèse d’un stress oxydatif au niveau des branchies d’ormeaux sensibles à la vibriose et 2/ une altération du fonctionnement des hémocytes, ce qui présume de l’une des stratégies majeures de virulence de V. harveyi.
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https://tel.archives-ouvertes.fr/tel-01124110/file/2013BRES0062.pdf BibTex
titre
Prey preferences of adult sea bass Dicentrarchus labrax in the northeastern Atlantic: Implications for bycatch of common dolphin Delphinus delphis
auteur
J. Spitz, Tiphaine Chouvelon, Marion Cardinaud, C. Kostecki, P. Lorance
article
ICES Journal of Marine Science, Oxford University Press (OUP), 2013, 70 (2), pp.452-461. ⟨10.1093/icesjms/fss200⟩
annee_publi
2013
resume
In the northeastern Atlantic, adult sea bass (Dicentrarchus labrax) is one of largest fish living on the shelf, and this species has important commercial value. However, pelagic trawl fisheries that target sea bass have negative operational interactions with common dolphins (Delphinus delphis). Our goal was to determine the diet of adult sea bass in the Bay of Biscay from stomachcontent and stable-isotope analyses, and explore the dietary overlap between sea bass and common dolphins. We found that sea bass primarily target small pelagic fish, most notably mackerel (Scomber scombrus), scads (Trachurus spp.), anchovy (Engraulis encrasicolus), and sardine (Sardina pilchardus). These four species also dominated the diets of common dolphins. This overlap in feeding preferences could increase the risk of dolphins being caught by trawl fisheries while feeding among sea bass, and may be an underlying mechanism to explain the high rate of common dolphin bycatch observed in the pelagic trawl fishery for sea bass in the Bay of Biscay. Understanding the foraging ecology and trophic interactions of predator species is an essential step for identifying and resolving management issues in the northeastern Atlantic and other marine ecosystems. © 2013 International Council for the Exploration of the Sea.
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titre
Gene expression patterns of abalone, Haliotis tuberculata, during successive infections by the pathogen Vibrio harveyi
auteur
Marie-Agnès Travers, Anne-Leila Meistertzheim, Marion Cardinaud, Carolyn S. Friedman, Sylvain Huchette, Dario Moraga, Christine Paillard
article
Journal of Invertebrate Pathology, Elsevier, 2010, 105 (3), pp.289-297. ⟨10.1016/j.jip.2010.08.001⟩
annee_publi
2010
resume
Since 1998, episodic mass mortality of the abalone Haliotis tuberculata has been observed along the northern Brittany coast of France caused by a complex interaction among the host, pathogen and environmental factors. In the present study, abalone were submitted to two successive infections with the pathogen Vibrio harveyi under controlled conditions. During the first challenge, infection by V.harveyi resulted in 64% mortality of mature abalone. After a second infection of those surviving the first challenge, only 44% mortality was observed. Physiological variability in the host response appears to be a major determinant in susceptibility to V.harveyi. In order to isolate differentially expressed genes in H.tuberculata challenged with this bacterium, suppression subtractive hybridization (SSH) cDNA libraries were constructed from muscle of moribund abalone (susceptibles), surviving individuals (apparently resistant to the bacterium) and control (unexposed) animals. Of the 1152 clones sequenced, 218 different partial cDNA sequences were obtained and represented 69 known genes. Of these, 65 were identified for the first time in H.tuberculata. Using real-time PCR, a time-course study was conducted on 19 of the genes identified by SSH. A majority of differentially expressed transcripts were down-regulated in susceptible individuals as compared to their resistant counterparts. Bacterial challenge of abalone resulted in the up-regulation of three transcripts (encoding ferritin, heat shock protein HSP84 and fatty acid binding protein FABP) in those that survived exposure to V.harveyi. This study has identified potential candidates for further investigation into the functional basis of resistance and susceptibility to summer vibriosis outbreaks in abalone.
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